性能
The quantity of free-circulating DNA in plasma, serum and other body fluids is usually low, and isolation is a challenge. QIAamp MinElute ccfDNA Kits provide excellent results with efficient purification of high-quality ccfDNA from serum and plasma samples, even when small amounts are present. Compared with other commercially available kits, QIAamp MinElute ccfDNA Kits recovered the greatest amount of ccfDNA.
Pre-concentration of ccfDNA on magnetic beads allows flexible and scalable handling of volumes from 1 to 10 ml while the use of a MinElute column allows flexible elution volumes between 20 μl and 80 μl, providing the highest possible concentration of nucleic acid species that are present in low concentrations (typically 1–100 ng/ml circulating DNA in human plasma).
The QIAamp MinElute ccfDNA Mini Kit has been optimized for sample volumes from 1 to 4 ml while the QIAamp MinElute ccfDNA Midi Kit has been optimized for sample volumes from 4 to 10 ml. QIAamp MinElute columns can bind fragmented nucleic acids that are as short as 20 bases, but yield depends on the sample volume and the concentration of circulating nucleic acids in the sample.
原理
Free-circulating DNA, such as tumor-specific extracellular DNA fragments in the blood or fetal DNA in maternal blood, is present in serum or plasma usually as short fragments of <1000 bp. Because ccfDNA may reflect the entire tumor genome, it has gained attention for its potential clinical utility, enabling use of “liquid biopsies” in the form of blood draws.
QIAamp MinElute ccfDNA Kits enable efficient purification of these circulating DNAs from human plasma or serum. The procedure can be partially automated on the
QIAcube.
操作流程
The kits use a fast procedure involving pre-concentration of circulating nucleic acids onto magnetic beads and a cleanup of the resulting pre-eluate on QIAamp MinElute columns in a quick and convenient spin procedure. The robust procedure helps to eliminate sample-to-sample cross-contamination and increases user safety when handling potentially infectious samples.
The simple procedure, which is highly suited for simultaneous processing of multiple samples, provides pure nucleic acids in less than 1 hour for 24 samples. Free-circulating DNA is eluted in Ultra-clean Water, ready for use in downstream reactions or storage a –30 to –15°C. Purified nucleic acids are free of proteins, nucleases and other impurities. The procedure can be partially automated on the
QIAcube.
应用
QIAamp MinElute ccfDNA Kits enable efficient purification of circulating DNAs from human plasma or serum.
