D310: Purity >99% (HPLC); alpha isomer <2%, nonionic detergent for membrane protein stabilization and crystallization

C₂₄H₄₆O₁₁

510.6

69227-93-6

CMC (H₂O): ~ 0.17 mM⁽¹⁾ (0.0087%)   
CMC (0.2 M NaCl): ~ 0.12 mM⁽²⁾
Aggregation Number (H2O)^(1-2): ~ 78-149
dn/dc (H₂O)⁽⁴⁾:   0.1435mL/gm
Micelle Size⁽⁵⁾: 72 kDa
Purity: ≥ 99% by HPLC analysis.
For molar volume check reference 3.
Percent alpha: < 2 (HPLC)
Percent dodecanol: < 0.005 (HPLC) 
pH (1% solution): 5-8
Solubility in water at 0-5°C: ≥ 20%
Conductance (10% solution): < 40 μS
Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
Absorbance of a 1% detergent solution: 
340 nm: < 0.02
280 nm: < 0.04
260 nm: < 0.06
225 nm: < 0.1

1.   VanAken, T., Foxall-VanAken, S., Castleman, S. and Ferguson-Miller, S. (1986) Methods Enzymol. 125, 27-35.
2.   Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
3.   Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E. (1970) Canadian J. Chem. 48, 2525-2531.
4.   Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract
5.   Strop, P., and Brunger, A.T. (2005) Protein Sci. 14, 2207-2211.